Communication between S1N330 and a region in S2 of murine coronavirus spike protein is important for virus entry into cells expressing CEACAM1b receptor.
Identifieur interne : 006430 ( Main/Exploration ); précédent : 006429; suivant : 006431Communication between S1N330 and a region in S2 of murine coronavirus spike protein is important for virus entry into cells expressing CEACAM1b receptor.
Auteurs : Shutoku Matsuyama [Japon] ; Fumihiro TaguchiSource :
- Virology [ 0042-6822 ] ; 2002.
Descripteurs français
- KwdFr :
- Animaux, Antigène carcinoembryonnaire, Antigènes CD, Cellules géantes, Cricetinae, Glycoprotéine de spicule des coronavirus, Glycoprotéines (métabolisme), Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Hémagglutinines virales (métabolisme), Lignée cellulaire, Molécules d'adhérence cellulaire, Mutation, Protéines de fusion virale (génétique), Protéines de fusion virale (métabolisme), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Récepteurs viraux (métabolisme), Réplication virale, Sites de fixation, Virus de l'hépatite murine (croissance et développement), Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (physiologie).
- MESH :
- croissance et développement : Virus de l'hépatite murine.
- génétique : Glycoprotéines membranaires, Protéines de fusion virale, Protéines de l'enveloppe virale, Virus de l'hépatite murine.
- métabolisme : Glycoprotéines, Glycoprotéines membranaires, Hémagglutinines virales, Protéines de fusion virale, Protéines de l'enveloppe virale, Récepteurs viraux.
- physiologie : Virus de l'hépatite murine.
- Animaux, Antigène carcinoembryonnaire, Antigènes CD, Cellules géantes, Cricetinae, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Lignée cellulaire, Molécules d'adhérence cellulaire, Mutation, Protéines de l'enveloppe virale, Réplication virale, Sites de fixation.
English descriptors
- KwdEn :
- Animals, Antigens, CD, Binding Sites, Carcinoembryonic Antigen, Cell Adhesion Molecules, Cell Line, Cricetinae, Giant Cells, Glycoproteins (metabolism), Hemagglutinins, Viral (metabolism), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Murine hepatitis virus (genetics), Murine hepatitis virus (growth & development), Murine hepatitis virus (physiology), Mutation, Receptors, Virus (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Viral Fusion Proteins (genetics), Viral Fusion Proteins (metabolism), Virus Replication.
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical , metabolism : Glycoproteins, Hemagglutinins, Viral, Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical : Antigens, CD, Carcinoembryonic Antigen, Cell Adhesion Molecules, Spike Glycoprotein, Coronavirus.
- genetics : Murine hepatitis virus.
- growth & development : Murine hepatitis virus.
- physiology : Murine hepatitis virus.
- Animals, Binding Sites, Cell Line, Cricetinae, Giant Cells, Mutation, Virus Replication.
Abstract
The soluble receptor-resistant (srr) mutants, srr7 and srr11, isolated from a murine coronavirus, mouse hepatitis virus (MHV) JHMV, have an amino acid mutation at positions 1114 (Leu to Phe) and 65 (Leu to His), respectively, in the spike (S) protein. These mutants failed to efficiently infect BHK cells expressing CEACAM1b (BHK-R2), due to their low entry into this cell line, although they infected cells expressing CEACAM1a (BHK-R1) in a manner similar to that of wild-type (wt) JHMV cl-2 (Matsuyama and Taguchi, Virology 273, 80-89, 2000). Following the repeated passage of these mutants through BHK-R2 cells, viruses were no longer isolated from srr11-infected cells, while two distinct mutants, srr7A and srr7B, were obtained from srr7-infected cells. Srr7A and srr7B grew 2 log10 higher than srr7 and induced fusion in BHK-R2 cells, being similar to wt virus. In addition to the amino acid change at position 1114 that stemmed from parental srr7, srr7A and srr7B had mutations around position 280, corresponding to the third region of the S1N330 receptor-binding site (S1N330-III) common to all MHV strains examined thus far. Srr7A and srr7B S proteins showed high fusogenicity in both BHK-R1 and BHK-R2 cells, like the wt virus, while srr7Aa and srr7Ba S proteins, which had mutations in S1N330-III but not at amino acid 1114, exhibited profoundly reduced fusion activity in these cell lines. These findings suggest that communication between S1N330-III and the amino acid at position 1114 is important for efficient fusion activity in BHK-R2 cells. S1N330-III is a possible region in the S1 involved in viral entry into cells.
DOI: 10.1006/viro.2002.1391
PubMed: 12033774
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 003462
- to stream PubMed, to step Curation: 003462
- to stream PubMed, to step Checkpoint: 003352
- to stream Ncbi, to step Merge: 000021
- to stream Ncbi, to step Curation: 000021
- to stream Ncbi, to step Checkpoint: 000021
- to stream Main, to step Merge: 006942
- to stream Main, to step Curation: 006430
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Communication between S1N330 and a region in S2 of murine coronavirus spike protein is important for virus entry into cells expressing CEACAM1b receptor.</title><author><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><affiliation wicri:level="1"><nlm:affiliation>National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502</wicri:regionArea><wicri:noRegion>187-8502</wicri:noRegion></affiliation></author><author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12033774</idno><idno type="pmid">12033774</idno><idno type="doi">10.1006/viro.2002.1391</idno><idno type="wicri:Area/PubMed/Corpus">003462</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003462</idno><idno type="wicri:Area/PubMed/Curation">003462</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003462</idno><idno type="wicri:Area/PubMed/Checkpoint">003352</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003352</idno><idno type="wicri:Area/Ncbi/Merge">000021</idno><idno type="wicri:Area/Ncbi/Curation">000021</idno><idno type="wicri:Area/Ncbi/Checkpoint">000021</idno><idno type="wicri:doubleKey">0042-6822:2002:Matsuyama S:communication:between:s</idno><idno type="wicri:Area/Main/Merge">006942</idno><idno type="wicri:Area/Main/Curation">006430</idno><idno type="wicri:Area/Main/Exploration">006430</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Communication between S1N330 and a region in S2 of murine coronavirus spike protein is important for virus entry into cells expressing CEACAM1b receptor.</title><author><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><affiliation wicri:level="1"><nlm:affiliation>National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502</wicri:regionArea><wicri:noRegion>187-8502</wicri:noRegion></affiliation></author><author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, CD</term><term>Binding Sites</term><term>Carcinoembryonic Antigen</term><term>Cell Adhesion Molecules</term><term>Cell Line</term><term>Cricetinae</term><term>Giant Cells</term><term>Glycoproteins (metabolism)</term><term>Hemagglutinins, Viral (metabolism)</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (metabolism)</term><term>Murine hepatitis virus (genetics)</term><term>Murine hepatitis virus (growth & development)</term><term>Murine hepatitis virus (physiology)</term><term>Mutation</term><term>Receptors, Virus (metabolism)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Fusion Proteins (genetics)</term><term>Viral Fusion Proteins (metabolism)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antigène carcinoembryonnaire</term><term>Antigènes CD</term><term>Cellules géantes</term><term>Cricetinae</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines (métabolisme)</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Hémagglutinines virales (métabolisme)</term><term>Lignée cellulaire</term><term>Molécules d'adhérence cellulaire</term><term>Mutation</term><term>Protéines de fusion virale (génétique)</term><term>Protéines de fusion virale (métabolisme)</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Récepteurs viraux (métabolisme)</term><term>Réplication virale</term><term>Sites de fixation</term><term>Virus de l'hépatite murine (croissance et développement)</term><term>Virus de l'hépatite murine (génétique)</term><term>Virus de l'hépatite murine (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glycoproteins</term><term>Hemagglutinins, Viral</term><term>Membrane Glycoproteins</term><term>Receptors, Virus</term><term>Viral Envelope Proteins</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antigens, CD</term><term>Carcinoembryonic Antigen</term><term>Cell Adhesion Molecules</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines</term><term>Glycoprotéines membranaires</term><term>Hémagglutinines virales</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Binding Sites</term><term>Cell Line</term><term>Cricetinae</term><term>Giant Cells</term><term>Mutation</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antigène carcinoembryonnaire</term><term>Antigènes CD</term><term>Cellules géantes</term><term>Cricetinae</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Lignée cellulaire</term><term>Molécules d'adhérence cellulaire</term><term>Mutation</term><term>Protéines de l'enveloppe virale</term><term>Réplication virale</term><term>Sites de fixation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The soluble receptor-resistant (srr) mutants, srr7 and srr11, isolated from a murine coronavirus, mouse hepatitis virus (MHV) JHMV, have an amino acid mutation at positions 1114 (Leu to Phe) and 65 (Leu to His), respectively, in the spike (S) protein. These mutants failed to efficiently infect BHK cells expressing CEACAM1b (BHK-R2), due to their low entry into this cell line, although they infected cells expressing CEACAM1a (BHK-R1) in a manner similar to that of wild-type (wt) JHMV cl-2 (Matsuyama and Taguchi, Virology 273, 80-89, 2000). Following the repeated passage of these mutants through BHK-R2 cells, viruses were no longer isolated from srr11-infected cells, while two distinct mutants, srr7A and srr7B, were obtained from srr7-infected cells. Srr7A and srr7B grew 2 log10 higher than srr7 and induced fusion in BHK-R2 cells, being similar to wt virus. In addition to the amino acid change at position 1114 that stemmed from parental srr7, srr7A and srr7B had mutations around position 280, corresponding to the third region of the S1N330 receptor-binding site (S1N330-III) common to all MHV strains examined thus far. Srr7A and srr7B S proteins showed high fusogenicity in both BHK-R1 and BHK-R2 cells, like the wt virus, while srr7Aa and srr7Ba S proteins, which had mutations in S1N330-III but not at amino acid 1114, exhibited profoundly reduced fusion activity in these cell lines. These findings suggest that communication between S1N330-III and the amino acid at position 1114 is important for efficient fusion activity in BHK-R2 cells. S1N330-III is a possible region in the S1 involved in viral entry into cells.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></noCountry><country name="Japon"><noRegion><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006430 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 006430 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:12033774
|texte= Communication between S1N330 and a region in S2 of murine coronavirus spike protein is important for virus entry into cells expressing CEACAM1b receptor.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:12033774" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |